Adherence of Pilus - Opa + Gonococci to Epithelial Cells h Vitro Involves Heparan Sulfate
نویسنده
چکیده
Neisseria gonorrhoeae attaches to host epithelial cells via pili and opacity-associated (Opa) outer membrane proteins. Pilusgonococci (Gc) of strain MS11 adhere to both human and nonhuman cells, but only when particular Opa proteins are expressed; OpaA + variants adhere best, OpaC § variants are next best, and the seven other Opa + variants adhere poorly or not at all. The adherence of OpaA + Gc to Chinese hamster ovary (CHO) cells is inhibited by heparin or heparan sulfate (HS), but not by chondroitin sulfate. OpaA + Gc do not adhere to CHO cells devoid of HS proteoglycans; low concentrations of heparin restore OpaA + Gc adherence to these HS-deficient CHO cells and high concentrations inhibit it. 3H-heparin binding to whole Gc parallels their adherence abilities (OpaA § > OpaC + > OpaH § >> Opas B, D, E, F, G, I = Opa= 0). Opa proteins separated by SDS-PAGE also bind 3H-heparin. These data suggest that adherence of pilus-, Opa + Gc involves HS-proteoglycan of eukaryotic cells.
منابع مشابه
Adherence of pilus- Opa+ gonococci to epithelial cells in vitro involves heparan sulfate
Neisseria gonorrhoeae attaches to host epithelial cells via pili and opacity-associated (Opa) outer membrane proteins. Pilus- gonococci (Gc) of strain MS11 adhere to both human and nonhuman cells, but only when particular Opa proteins are expressed; OpaA+ variants adhere best, OpaC+ variants are next best, and the seven other Opa+ variants adhere poorly or not at all. The adherence of OpaA+ Gc ...
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